Product
Promotional Offer on SpermCryoProtec II
BioTipp in association with NidaCon International AB are offering you a 50% discount on the list price on Sperm CryoProtec II, together with our new cryo device and Lab. instructions
Cat. Number: SCP-020
Minimum Order 5 pcs.
Please quote: WEB-0103
This offer is Valid until 31st March 2009
Product Summary
Sperm CryoProtec™ II is a sterile salt solution containing glycerol, optimised for freezing human sperm.
Performance Characteristics
pH: 7.5 - 8.5
Endotoxin levels
< 1.0 EU/mL
Recovery rate of the original motile spermatozoa after freezing and thawing
>50%.
Contents tested by human sperm survival only.
Bottles and stoppers are 2-cell MEA tested.
Components
- Sodium chloride
- Magnesium sulphate
- Potassium dihydrogen phosphate
- Glycerol
- Sodium bicarbonate
- H2O
- Potassium chloride
- Glucose
- Lactate
- Pyruvate
- EDTA
- HEPES
Storage and Stability
Store at 2 to 30ºC and avoid temperatures above or below these values. Under these conditions Sperm CryoProtec™ II has a shelf-life of 12 months. The expiry date is shown on both bottles and cartons.
Open and close bottles under aseptic conditions. After opening store at 2 to 8ºC when not in use. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
No antibiotics, unstable additives or preservatives have been added by the manufacturer to Sperm CryoProtec™ II.
Precautions and Warnings
• Use aseptic procedures at all times
• Sperm CryoProtec™ II contains glycerol which is combustible. A material safety data sheet is available from the distributor or manufacturer (see nidacon.com)
• Do not use any solution which shows evidence of bacterial contamination
• Do not use contents if tamper-evident seal is broken
• Federal Law (USA) restricts this device to sale by or on the order of a physician
• Please check for regulatory compliance governing the use of ART products in your country
Recommendations
Although it is possible to freeze unprocessed semen, we recommend that the ejaculate be prepared on a PureSperm® density gradient and washed with PureSperm®Wash before adding Sperm CryoProtec™ II. This method removes seminal plasma as well as ROS and their sources, thereby ensuring optimal recovery of motile sperm on thawing.
Reagents and Equipment
• Sperm CryoProtec™ II and PureSperm®Wash
• Sterile pipettes
• Disposable sterile conical centrifuge tubes
(e.g. Falcon 2075)
• Disposable sterile cryopreservation vials or plastic straws
Ordering Information
Article No. Name Volume
SCP-020 Sperm CryoProtec™II 2 × 20 mL
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