An alternative method, the UniLayer filtration of semen, of preparing human sperm for IUI
Many clinicians in small clinics or private practices are looking for a safe and effective method of preparing sperm for intra-uterine insemination. They are unsure of their ability to layer a density gradient correctly to achieve optimal results. However, they also know that a “swim up” does not remove sperm with abnormal morphology or damaged DNA, and does not separate out bacteria and viruses from the sperm preparation.
Features:
Now there is an alternative method of preparing human sperm for IUI – the PureSperm SpeediKit. The kit contains sufficient sperm preparation materials for ten patients, already dispensed in Type 1 glass centrifuge tubes.
Components:
- Sodium chloride
- Potassium chloride
- Magnesium sulphate
- Potassium dihydrogen phosphate
- Sodium bicarbonate
- EDTA
- hSA (human Serum Albumin)
- Sodium citrate
- Glucose
- Calcium lactate
- Sodium pyruvate
- HEPES
- Purified water
- Silane-coated silica.
Performance Characteristics:
- pH of PureSperm UniLayer: 7.4 – 7.8.
- pH of PureSperm Wash: 7.3 – 8.5.
- Osmolality of PureSperm UniLayer (mOsm/kg H2O): 300 – 330.
- Osmolality of PureSperm Wash (mOsm/kg H2O): 290 – 300.
- Endotoxin levels (EU/mL): <1.0.
- Contents are tested by human sperm survival only.
- Tubes and stoppers are M.E.A. tested.
Storage and Stability:
- Store at 2 to 27ºC and avoid temperatures above or below these values. Under these conditions the contents of the kit have a shelf-life of minimum 12 months from production. The expiry date is shown on both tubes and cartons. Shelf-life on the product label applies when the product is stored according to manufacturer’s recommendations.
- No antibiotics, unstable additives or preservatives have been added by the manufacturer to PureSperm SpeediKit.
Precautions and Warnings:
- When retrieving the sperm pellet, follow the instructions given in the pack insert to avoid inadvertent contamination.
- Use aseptic procedures at all times.
- If available, use sealed buckets during centrifugation to avoid creation of aerosols.
- Clean accidental spills using a dampened cloth or paper. PureSperm SpeediKit causes floors and benches to be extremely slippery.
- PureSperm SpeediKit does not represent any kind of fire or combustion hazard. A material safety data sheet is available from the distributor or manufacturer.
- Do not use any solution which shows evidence of bacterial contamination.
- Do not use contents if tamper-evident seal is broken.
- Federal Law (USA) restricts this device to sale by or on the order of a physician.
- Please check for regulatory compliance governing the use of ART products in your country.
To order contact us at:
Ireland Head Office: +353 (0)52 744289645
4-5 The Square , Cahir Co. Tipperary Ireland
E-mail: info@biotipp.com
